Cre/loxP system
Aire遺伝子のGFPノックインマウス。エクソン1と2の間にGFPが挿入されている。
RBRC03515
Aire(GFP) (Acc.No. CDB0479K)
Aire(GFP) (Acc.No. CDB0479K)
C（3〜6か月）
true
RIKEN CDB
(1) The RECIPIENT shall obtain a prior agreement from Dr. Mitsuru Matsumoto (mitsuru@ier.tokushima-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. (CDB0479K) (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) and the BIOLOGICAL RESOURCE is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (J Exp Med. 2008 205(12):2827-38.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
RIKEN CDB
条件を付加する。<br>(1) 分与希望者は事前に 松本 満 (mitsuru@ier.tokushima-u.ac.jp) に連絡をとり、分与に関して許可を得ること。(2) 当該マウスを用いる論文発表に際してはAccession No. CDB0479K、そのWEB サイトhttp://www2.clst.riken.jp/arg/mutant%20mice%20list.htmlとともに当該マウスを記載すること。(3) 当該マウスを用いる論文発表に際しては、その由来として第一報論文 J Exp Med. 2008 205(12):2827-38. を引用すること。（4）発表論文を寄託者（mutant.bdr@riken.jp）に連絡すること。
Fluorescent Proteins/lacZ System
B6.Cg-Aire<tm2Mmat>/Rbrc
B6.Cg-Aire<tm2Mmat>/Rbrc
<a href='https://brc.riken.jp/mus/pcr03515'>Genotyping protocol -PCR-</a>
理化学研究所発生・再生科学総合研究センター・動物資源開発室、徳島大学疾患酵素学研究センター・松本 満先生。TT2 ES細胞由来。
Aire GFP knock-in mice. A GFP was inserted in the Aire gene locus between exon 1 and 2.
Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li><li>GFP Transfer License (<A HREF="https://web.brc.riken.jp/ja/method/link/gfp_conclude">Japanese</A> / <A HREF="https://web.brc.riken.jp/en/method/link/gfp_conclude">English</A>)<br>Please fill in the <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_a.doc">Schedule A</A>, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_b.doc"> Schedule B</A>. </li></ol>
C (3-6 months)
TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Phage P1 loxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Neo, jellyfish GFP cDNA, mouse Aire genomic DNA
Developed by Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology and Mitsuru Matsumoto, Institute for Enzyme Research, University of Tokushima. TT2 ES cells were used.